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The different steps in the IVF avenue, are illustrated in the graphics below by Professor Poul Hyttel who is also on the ARTSMedia scientific board.
AM-IVC Medium with Human Serum Albumin
10 ml glass bottles, individually packed, provided sterile.
ARTSMedia In Vitro Culture Medium is a bicarbonate-buffered balanced salts solution, with Physiological Salts, Amino Acids, Calcium Lactate Pentahydrate, EDTA, Gentamicin Sulphate 10 μg/ml, Glucose, L-glutamine, Human Serum Albumin (HSA), Insulin, Sodium Hyaluronate, Sodium Pyruvate, Sodium Bicarbonate, and Phenol Red.
AM-IVC Medium is bicarbonate buffered and should pre- equilibrate in a CO₂ incubator set at 37°C and 5-6% CO₂. Note: Adjust incubator settings to a higher CO₂ concentration if the altitude is above sea level.
To maintain pH, temperature, and osmolality, make sure that the medium is completely covered with oil.
Physiological salts, Potassium chloride, Magnesium sulfate, Potassium phosphate monobasic, anhydrous, Magnesium chloride, Sodium chloride, Calcium chloride, Synthetic serum replacements, Sodium bicarbonate, Human insulin recombinant, Sodium selenite, Ethanolamin, EDTA, EDTA, FE3+ iron(III) sodium salt, Sodiumcitrate, Zink sulphate, Gentamicin, Glycine, L-aspartic acid, Amino acids, Alanyl-L- glutamine, Vitamins, Myo inositol, Calcium lactate, Glucose, Sodium pyruvate, HSA, Hyaluronic acid, Phenol red
AM-IVC Medium is a culture medium intended for in vitro fertilization and in vitro culture of human gametes and embryos from fertilization until the blastocyst stage of development (day 5). The medium can also be used for embryo transfer.
Store dark at 2-8°C in original container. Do not freeze.
pH: 7,2 – 7,45 (Ph Eur. 2.2.3, USP <791>)
Osmolality: 257-275 mOsm/kg (Ph Eur. 2.2.35, USP <785>)
Appearance: Clear and particulate free
Sterility: No growth (Ph Eur. 2.6.1, USP<71>)
Endotoxins: < 0.05 EU/ml (USP<85>)
1-cell Mouse Embryo Assay (MEA) ≥ 80% embryos
developed to expanded blastocysts at 96 hours.
The results of each batch are stated on Certificate of Analysis, which is available upon request.
The AM-IVC medium is available for sale in USA, and for other regions in the rest of the world get in touch info@artsmedia.dk
AM HV-Oil is intended for use in In Vitro Fertilization (IVF), Intracytoplasmic Sperm Injection (ICSI) and related Assisted reproductive technologies (ART) as overlay on media.
Keep away from sunlight at 15-25°C in original container. Do not freeze.
Density: 0.86-0.88g/ml
Viscosity: 95-140cP at 30 °C
Endotoxin : < 0.1EU/ml USP <85>
Peroxide Value (POV): < 0.1mEq/kg
Sterility : No growth (Ph. Eur. 2.6.1, USP<71>)
1-Cell Mouse Embryo Assay: ≥ 80% blastocysts embryos
developed to expanded blastocyst at 120 hours
The results of each batch are stated on Certificate of Analysis, which is available upon request.
The AM-HV oil is available for sale in USA, and for other regions in the rest of the world get in touch info@artsmedia.dk
50 ml and 100 ml glass bottles, individually packed, provided sterile. Peroxide tested pre-washed oil washed and ready-to-use mineral oils for use in human assisted reproductive techniques.
Oil viscosity influences temperature, pH and osmolarity, with higher viscosity demonstrating higher yields after overlay in embryo culture in terms of blastocyst formation rates and cell counts.
Advantages: easier to handle, droplets are maintained better, and oil doesn’t spill over as easy.
Temperature, pH is better maintained and increased media osmolarity is avoided both during handling and during culture.
The unit of viscosity is newton-second per square metre, which is usually expressed as pascal-second in SI units, cP.
We have increased the viscosity from < 30 cP to 95-140 cP.
Quality Control: we still apply the strict quality control tests of each batch which besides a bovine embryo assay (BEA) test also includes *) POV, sterility, and endotoxin tests.
*) The peroxide value (POV) is defined as the reactive oxygen contents expressed in terms of milliequivalents (meq) of free iodine per kilogramme of fat. It is determined by titrating iodine liberated from potassium iodide with sodium thiosulphate solution. Oils with POV well below 10 meq/kg are considered fresh – The limit for our oil is <0.1meq/kg
The oil should be pre-warmed prior to use.
To maintain pH, temperature, and osmolality, make sure that the medium is completely covered with oil.
The oil should be used according to the established procedures and protocols optimized for the individual clinic.
AM-HV Oil is a pharmaceutical grade light mineral oil*), pre-washed and sterile filtered light paraffin oil. Oil is often the key to poor results, use only BEA tested and Peroxide Value (POV) tested oil as minimum standard quality control.
*) Liquid paraffin oil is a mineral oil and is a by-product of crude oil distillation.
AM-SDG Medium 45 %
AM-SDG Medium 45 % and AM-SDG Medium 90 % is a ready to use two layer gradient system designed for separating human sperm using density gradient centrifugation. The AM-SDG media system is suitable for semen preparation for Intra Uterine Insemination (IUI), In Vitro Fertilization (IVF), and IntraCytoplasmic Sperm Injection (ICSI).
pH: 7,2 – 7,9 (USP <791>)
Osmolality: 310-340 mOsm/kg (USP <785>)
Density: 1.0500-1.0700 g/ml
Sterility: No growth (USP<71>)
Endotoxins: < 0.5 EU/ml (USP<85>)
HSSA after 4 hours > 80 %
HSSA after 24 hours > 75 %
AM-SDG Medium 90 %
AM-SDG Medium 45 % and AM-SDG Medium 90 % is a ready to use two layer gradient system designed for separating human sperm using density gradient centrifugation. The AM-SDG media system is suitable for semen preparation for Intra Uterine Insemination (IUI), In Vitro Fertilization (IVF), and IntraCytoplasmic Sperm Injection (ICSI).
pH: 7,2 – 7,9 (USP <791>)
Osmolality: 320-350 mOsm/kg (USP <785>)
Density: 1.1050-1.1150 g/ml
Sterility: No growth (USP<71>)
Endotoxins: < 0.5 EU/ml (USP<85>)
HSSA after 4 hours > 80 %
HSSA after 24 hours > 75 %
AM-SDG Medium 90 % are available for sale in USA, and for other regions in the rest of the world get in touch info@artsmedia.dk
20 ml, 50 ml, and 100 ml glass bottles, individually packed, provided sterile. The AM-SDG media system is a HEPES-buffered medium with suspended silane-coated colloidal silica particles.
Silane-coated colloidal silica particles, Sodium Chloride, Hepes, Glucose, Sodium Hydrogen Carbonate, Potassium Chloride, Calcium Chloride, Phosphate, Magnesium Sulphate, Sodium Pyruvate
Tel: +45 53504530
Mail: info@artsmedia.dk
We are in the process of registering our new product portfolio within the different markets.
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