Embryos do not have the same metabolic needs throughout development. This is the foundation behind sequential media systems. However, from extensive experience and many tests a well-designed one-step medium consistently outperformed sequential protocols.
Recent research underscores the benefits of using bicarbonate-buffered media during ICSI, demonstrating improved fertilization rates, reduced abnormal fertilization, and lower chromosome mosaicism compared to zwitterionic buffers like HEPES. Bicarbonate, as a physiological buffer, maintains pH and provides nutritional support for oocytes, enhancing embryo development. Clinics are advised to adopt bicarbonate buffers and optimize preparation practices for better outcomes in assisted reproduction.
Using non-IVF approved plastic dishes can harm embryo development and reduce blastocyst rates. IVF-grade plastics undergo strict quality control through mouse embryo assay (MEA) testing, ensuring minimal toxicity. Avoid using non-IVF grade plastics for media storage or in incubators, as they can compromise media and create a toxic environment. Always prioritize IVF-certified disposables for optimal embryo culture.